Part:BBa_K3015006:Design

T7 RNA Polymerase (No BbsI/BpiI)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The T7-polymerase will start transcription from a T7-promotor

We Mutated the following basepairs to delete BbsI/BpiI recognition sites
1) S202 changed from TCT to TCC (bp 604-606)
2) E207 changed from GAA to GAG (bp 619-621)
3) E309 changed from GAA to GAG (bp 925-927)
4) E365 changed from GAA to GAG (bp 1093-1095)
5) K714 changed from AAG to AAA (bp 2140-21429

Source

T7 polymerase from BBa_K145001